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The Negative is unlocked by beating Sheol 5 times. It looks like an inverted polaroid. Once you defeat Mom with this unlocked, you will have the option of choosing The Polaroid or The Negative. The Negative is activated when you have a half (or less) of a red heart like The Polaroid, but instead of giving a temporary shield, it damages all enemies in the room for a set amount of damage, similar to the Necronomicon. The Negative will also allow you to go to The Dark Room.