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The Dark Room is accessed by opening the chest at the end of Sheol (just like at the end of the cathedral with the Polaroid), only if the player has picked up The Negative instead of the Polaroid. Similar to The Chest, rooms usually contain multiple bosses, but the final boss of the floor will always be The Lamb. The Dark Room can also be accessed through a Sheol Black Market trapdoor, or the trapdoor in an I AM ERROR room.
The spawn room always contains four Red Chests, and every subsequent Golden Chests found on the floor will always contain an item, unless it's a red one. After completing The Dark Room for the first time, the spawn room will also contain the golden door that leads to the Mega Satan fight.
|Normal & Hard Mode|
|The Basement • The Cellar • Burning Basement|
|The Caves • The Catacombs • Flooded Caves|
|The Depths • Necropolis • Dank Depths|
|The Womb • Utero • Scarred Womb|
|Sheol • Cathedral • Blue Womb|
|The Dark Room • The Chest|
|The Basement • The Caves • The Depths • The Womb • Sheol • The Shop • Ultra Greed|