The Christian scriptures, containing 73 books of the New and Old Testament.
Upon activation, Isaac grows angel wings and gains the ability to fly for that room. Using the Bible on Mom, Mom's Heart or It Lives will result in an instant kill. Using the Bible on Satan, Isaac or ???, will deal an exceptional amount of damage to the player, usually resulting in death, but death can be prevented with soul hearts or The Wafer.
- The player's wings turn black if Lord Of The Pit, Spirit Of The Night, The Pact or Brimstone have been picked up or if The Nail or Whore of Babylon have been activated.
- The Rosary allows for this collectible to be found with high frequency in the shop.
- This item will be completely useless when used (except against Mom and Mom's Heart/It Lives!) if Isaac already has Flight.
- As with most Activated Items, Items such as Habit, 9 Volt and The Battery decrease the charge time, increasing the effectiveness of this item.
- Using this item to kill Mom or Mom's Heart unlocks The Halo.
- Using the Bible in the boss rooms of Satan, Isaac or ??? will always result in damage to Isaac, regardless of whether that boss is alive or not. (May be intentional)