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They are only found in The Basement from the original game. In Rebirth, they are commonly found in The Cellar, The Caves, The Catacombs, The Depths, and Necropolis. In the Afterbirth expansion, they can be also found in new alternative floors such as Burning Basement.
BehaviorPooters move around the room randomly, instead of following Isaac. They fire a single blood shot at Isaac when he gets in their line of sight. Like Black Flies, they cannot deal a contact damage to Isaac.
- They can be spawned by The Wretched, Gurdy, Famine, The Duke of Flies, Mulligans, Swarmers, Hives and Mom.
- The word "Pooter" is a device used in the collection of insects, crustaceans or other small, fragile organisms, usually for scientific purposes.