Pooters move around the room randomly, instead of following Isaac. They fire a single blood shot at Isaac when he gets in their line of sight.
Pooters do not deal contact damage.
A variant of Pooters called the Fat Fly was added in the Wrath of the Lamb expansion. They can be naturally found in Wrath of the Lamb floor such as the Catacombs, and can replace Pooters where ever they spawn.
They behave identically to regular Pooters, but shoot two projectiles in a V-pattern similar to that of the Keeper.
Although Fat Flies' projectiles can easily be avoided by going in between them, dodging multiple attacks from them can prove difficult as they often appear in groups.
All champion varities have double health and do double damage.