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She is a pink version of Isaac under the influence of The Virus.
She's quite fast and attacks by trying to deal contact damage, but has no other attacks.
StrategiesAs stated above, Lust has no attacks other than simply chasing Isaac, which can be much more threatening than it seems. If Isaac has no speed boosts, or has even lost speed, Lust can be a very difficult boss. The best way to fight Lust seems to be to break sightlines as much as possible. Lust slows down to a "searching" mode when she loses sight of Isaac, and this can be used to add distance between the player and Lust. Using the spawned rocks and quickly turning corners can conserve hearts and make this fight easier. Use of bombs can block her movement, as she is unable to push them away, but also won't walk around them.
Tears that induce slowness (including Ball of Tar and Little Gish) or fear (including Mom's Perfume and Mom's Bra) prove effective in negating Lust's only method of dealing damage. Another way to defeat her with flight and/or spectral tears; this allows for a unique strategy in which a player flies directly over rocks and fires at Lust as she pursues. Due to Lust having no way to destroy the rocks, she'll have no way to attack the player.
- Lust has been confirmed to be female by Edmund McMillen.
- Lust, having the same ability and the chance to drop The Virus, is most likely a reference to STDs.
- Lust's sprite is in fact just a recolor of Isaac equipped with The Virus.
- Lust dropping Pills is most likely a reference to birth control pills.
- Lust's attack (chasing after the player) may be a sign of love for the player, hence, running after Isaac.