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A miniature version of the boss Gish.
Follows Isaac around shooting tar projectiles that reduce enemies' movement speed, shot speed, and range for 2.5 seconds.
- Spider's Bite - Enemies that are hit by both Little Gish's projectiles and Isaac's tears will be greatly slowed, making them much easier to hit.
- Gish is a character from Edmund's game, Gish. Gish was also an unlockable in another of Edmund's games, Super Meat Boy and the free flash game Meat Boy, and appeared as a part of customization in his game, Host.