Keeper may refer to:
- Greed - A miniboss and one of the Seven Deadly Sins.
- Super Greed - A stronger version of the normal Greed.
- Ultra Greed - A boss version of Greed added to the Afterbirth DLC.
- Shopkeeper - A non-playable character that can be found in Shop rooms.
- Key Beggar - A type of Beggar.
- Keeper (character) - A playable character in the Afterbirth DLC for The Binding of Isaac: Rebirth.
- Old Man Keeper - An unused character for the Afterbirth DLC.
- Head Of The Keeper - An item added to the Afterbirth DLC.