For information on the character, see Isaac.
Isaac will appear lying down in the center of the room, crying on the floor. He shoots 12 high-velocity tears in a radial burst and will retaliate with streams of tears when hit.
At 66% of his health, a light will shine on Isaac, and he jumps from the ground. Isaac will then start shooting 4 large purple tears, which home slightly, in all directions. He will still retaliate with some tears when hit.
Triggered near 33% of Isaac's health. Isaac will grow angel wings, smile, and start floating. White beams will be randomly shot down from the sky (Similar to Crack The Sky and White Pony), Angel Babies will start spawning frequently (up to a maximum of 6), and the same homing projectiles from before will be shot in six directions at the same time. At this point, Isaac will no longer retaliate with tears when hit.
- Using The Bible during or after the fight will instantly kill the player (Similar to ??? and Satan) unless he/she has The Wafer item.
- Using items with a high fire rate (Such as Technology 2) is dangerous in this fight, as Isaac will retaliate with a very large amount of tears upon being hit. This also applies to effects which apply damage rapidly, such as Mom's Knife.
- Applying slow effects such as Little Gish or Spider Bite to Isaac will not affect the speed of his homing tears, but will slow all normal tears.
- Activating Mom's Bra or triggering the special effect of Mom's Contacts will prevent Isaac from spawning enemies or attacking at all—including when damaged—for the duration of the effect.
- Isaac cannot be damaged by the Crack The Sky light beams, or by his own. However, any Angel Babies in the fight can take a small amount of damage from his light beams.
- If the player uses The Hourglass, Isaac will fire blood tears instead of the usual blue tears and purple homing tears.
- If the player is killed by the light beams in the final stage of the battle, the Last Will will display a picture of War.
- The third form is referenced in the DLC's trailer, which showed Isaac sprouting wings.
- When in Phase 1, Isaac makes the same weeping noises as the Mulligan.
- When Isaac was first added in the initial release of Wrath of the Lamb, his third form only consisted of the rays of light, except with a tremendous rate of firing.
- The picture of Isaac on Isaac's Last Will is identical to that of the Mulligan, but without the bloated head.
|Basement||Larry Jr. • Monstro • The Duke of Flies • Gemini • Steven • Famine|
|Cellar||Widow • Pin • The Duke of Flies • Famine • Gurdy Jr. • The Blighted Ovum|
|The Caves||Fistula • Peep • Gurdy • Chub • C.H.A.D. • Pestilence|
|Catacombs||The Husk • The Hollow • The Blighted Ovum • The Carrion Queen • Peep • Pestilence • The Wretched • Gurdy Jr.|
|The Depths||Loki • Monstro II • Gish • War • Mom|
|Necropolis||Mask of Infamy • Daddy Long Legs • The Carrion Queen • The Bloat • Loki • War • The Wretched • Mom|
|The Womb||Scolex • Blastocyst • The Bloat • Death • Conquest • Daddy Long Legs • Triachnid • Teratoma • Mom's Heart • It Lives|
|Utero||Teratoma • The Bloat • Death • Conquest • Daddy Long Legs • Triachnid • Lokii • Mom's Heart • It Lives|
|Sheol||The Fallen • Satan|
|First to fifth and seventh level||The Fallen • Headless Horseman|