The vial will break when Isaac receives damage and a blue puddle of Creep will appear that can damage enemies, similar to Lemon Mishap. Until the vial reforms, upon taking damage, no new Creep will appear.
The vial is reformed upon entering a different room.
- Holy water is water that has been blessed by a saint or clergyman. It is used to confer blessings upon believers, as well as baptism.
- The design of the item comes from how the Holy Water item appeared in Castlevania.