Flies are small flying insects that appeared in The Binding of Isaac: Rebirth. They may refer to:
- Black Fly - A very common black-colored fly enemy.
- Attack Fly - A red/black flashing fly enemy.
- Moter - A pair of Attack Flies joined together.
- Eternal Fly - A pure white-colored fly enemy.
- Pooter - A bloated version of normal flies.
- Fat Fly - A fatter and stronger version of the Pooter.
- Boom Fly - A larger version of normal flies that can explode upon death.
- Red Boom Fly - A red-colored Boom Fly.
- Drowned Boom Fly - A blue-colored Boom Fly. (?)
- Sucker - More powerful version of normal flies.
- Spit - A green-colored Sucker.
- Swarm Fly - A yellow-colored fly. (?)
- Dart Fly - A type of fly looking similar to that of the Swarm Fly. (?)
- Ring Fly - A pink-colored fly.
- Level 2 Fly - A much stronger variant of normal flies.
- Full Fly - A powerful version of normal flies related to Level 2 Flies.
- Hush Fly - A bluer-colored fly. (?)
- The Duke of Flies - The boss of The Basement and The Cellar.
- The Husk - A posthumous counterpart of The Duke of Flies.
- Forever Alone - A fly familiar item that orbits around Isaac.
- Papa Fly - A familiar/passive item. (?)
- Pretty Fly - A collectible passive item.
- Beelzebub - A transformation based on the demon of the same name being titled as "lord of the flies".