Title Screens[]
All title screens in the normal version of the game (NOTE: You can get all of these in Wrath of the Lamb as well.)
The default title screen.
After first Mom's Heart kill.
After six Mom's Heart kills.
After nine Mom's heart kills.
After ten Mom's Heart kills.
After you've collected all items in the game.
Wrath of the Lamb[]
After beating the Cathedral for the first time.
After beating the Cathedral for the second time.
After beating the Cathedral the third time.
After beating the Cathedral for the fourth time.
After beating the Cathedral for the fifth time.
After beating ??? in the Chest, or previously, beating Isaac with the Polaroid.
Atfer you've unlocked every item in the game (including all WoTL items) and unlocked every achievement.
Eternal Edition[]
After beating Hard Mode with all characters and unlocking all Hard Mode achievements