Ace of Spades is a trinket first added to the Wrath of the Lamb DLC for the original game, and also introduced in the Rebirth remake. It is a white card printed with a spade symbol.
- Increases the chance of Tarot Cards or Suit Cards dropping after clearing a room, and the chance of finding Cards when opening a Chest.
- Chance of finding a Card when clearing a room is increased by 10%.
- Chance of finding a Card when opening a Chest is increased by 66%.
- In real-life, the Ace of spades card is found many times on the battlefield, especially American soldiers.